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KMID : 0367420040470090992
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Journal of Korean Pediatric Society
2004 Volume.47 No. 9 p.992 ~ p.1001
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Febrile Seizure-induced Neuroexcitability in Immature Rat Hipocampus.
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Choi Bong-Keun
Hwang Kyu-Geun
Bae Hae-Rahn
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Abstract
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To determine whether febrile seizure enhances neuroexcitability by altering synaptic transmission and whether febrile seizure-induced hyperexcitability leads to long-lasting neuronal death.
Methods : We investigated the expression of synaptic and postsynaptic proteins and the apoptosis of neuronal cells in rat pup hippocampus after hyperthermic seizure using immunoblotting and confocal microscopy.
Results : Hyperthermic seizure enhanced the long-term expressions of presynaptic proteins such as syntaxin, VAMP, SNAP-25 and nSec1, whereas that of NSF was decreased. The expressions of postsynaptic NMDA receptors 1, 2a and 2b were up-regulated. The expression of postsynaptic AMPA glutamate receptors 1 month after hyperthermic seizures altered by way of increasing the ratio of GluR1 to GluR2 and decreasing NSF-GluR2 interaction, which leads to the formation of Ca2＋ permeable AMPA receptors and enhanced toxicity. However, in spite of enhanced neuroexcitability, there was a transient increase of neuronal death in hipocampus one week after hyperthermic seizure, but returned to baseline one month later.
Conclusion : These results demonstrate both presynaptic and postsynaptic forms of long-term enhancement of glutamate synaptic transmission after hyperthermic seizure and support the idea that early-life febrile seizure might have persistent effects on neuronal excitability in the hippocampus. (Korean J Pediatr 2004;47:992-1001)
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KEYWORD
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Seizure, Febrile, Rat, Synaptic transmission, Receptor, AMPA
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